Journal: PLoS ONE

Article Title: Cell Population Kinetics of Collagen Scaffolds in Ex Vivo Oral Wound Repair

doi: 10.1371/journal.pone.0112680

Figure Lengend Snippet: The ex vivo wound healing model is shown in top view and cross-sectional view (A). The bottom of the well was covered with an acellular growth factor reduced basal membrane extract (BME). The scaffold (sponge type scaffold composed of cross-linked collagen or the gel type scaffold composed of rat collagen I) in the center was surrounded by a BME-gel containing 800,000 hGFs/ml and covered with an acellular BME gel and Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 1% fetal bovine serum (FBS) and antibiotics. Both scaffold and gel were tested with and without the addition of platelet-derived growth factor-BB (PDGF). Fluorescence images were taken to assess DiI-labeled cells within the scaffold over time. To assess the metabolic activity and gene expression dynamics, the scaffolds including the “active zone” (dashed line) were subjected to MTT tests, gene array analysis, and quantitative PCR. (B) Representative scanning electron microscopy images depict the morphology of the collagen gel, collagen scaffold at 400×, and 800× magnification. The white bar represents 100 µm.

Article Snippet: 48 well plates were first layered with 50 μL of 12 mg/ml growth factor reduced basal membrane extract (BME; CULTREX, TREVIGEN, Gaithersburg, MD, USA) without cells.

Techniques: Ex Vivo, Membrane, Modification, Derivative Assay, Fluorescence, Labeling, Activity Assay, Gene Expression, Real-time Polymerase Chain Reaction, Electron Microscopy

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 1. Liver-specific FGF21 knockout abrogated OVX-induced central obesity in mice. (A) Body weight profile of mice following OVX or the combination of OVX plus liver-specific FGF21 knockout. (B) The serum FGF21 concentrations in mice at decapitation. (C) Representative figures of mice indicating visceral adipose deposition at decapitation. (D) The average visceral fat weight of mice from each group. (E) Representative H&E staining of visceral adipose tissues of mice at decapitation. In figure A: * denotes p < 0.05 (OVX versus Sham/OVX+FGF21 LKO); in figure B and D: ** p < 0.01; *** p < 0.001, NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out, Staining

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 2. Liver-specific FGF21 knockout failed to rescue OVX-induced dyslipidemia and hepatic steatosis in mice. (A) Serum concentration of triglyceride (TG). (B) Serum concentration of free fat acid (FFA). (C) Liver weight. (D) Liver TG content. (E) The representative H&E staining of liver tissues from each group. Arrows indicate lipid droplets. * p < 0.05; ** p < 0.01; *** p < 0.001; NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out, Concentration Assay, Staining

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 3. Liver-specific FGF21 knockout exacerbated OVX-induced glucose metabolic abnormalities in mice. (A) Glucose tolerance test (GTT). (B) The area under the curve of GTT. (C) Insulin tolerance test (ITT). (D) The area under the curve of ITT. (E) Pyruvate tolerance test (PTT). (F) The area under the curve of PTT. Note figure (A,C,E): * OVX+FGF21 LKO versus Sham (p < 0.05), φ OVX+FGF21 LKO versus OVX (p < 0.05), # OVX versus Sham (p < 0.05); figure (B,D,F): * p < 0.05; ** p < 0.01; ***p < 0.001; NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 4. Transcriptomic profiling revealing Hsd11b1 plays a central role in mediating FGF21 LKO on abrogating OVX-induced central obesity in mice. (A) Venn diagram of differentially expressed genes (DEGs) among groups. (B) The number of DEGs between pairwise groups. (C) The recovered DEGs (rDEGs) in OVX mice following FGF21 LKO. rDEGs are genes whose expression in OVX was significantly different from the sham controls but recovered back to the sham levels after FGF21 LKO. (D) The heat map of rDEGs involved in lipid metabolism process (GO:0006629). Red lines indicate DEGs between OVX+FGF21 LKO versus OVX. (E) Gene set enrichment analysis (GSEA) showed FGF21 LKO reduced adipogenesis in OVX mice, and leading-edge gene analysis highlighted that Hsd11b1 plays a major role in mediating FGF21 LKO on preventing adipogenesis in OVX mice.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 5. FGF21 LKO reversed circulating high corticosterone but not high FSH in OVX mice. (A) Serum concentration of FSH. (B) Serum concentration of corticosterone. (C) Effects of transient recombinant FGF21 replacement on serum concentration of corticosterone in OVX+FGF21 LKO mice. (D) Effects of transient recombinant FGF21 replacement on visceral adipose Hsd11b1 expression in OVX+FGF21 LKO mice. * p < 0.05; ** p < 0.01; *** p < 0.001; NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Concentration Assay, Recombinant, Expressing

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 6. FGF21 LKO reduced serum insulin levels in OVX mice. (A) Serum concentration of insulin; (B) Gene set enrichment analysis indicated FGF21 LKO reduced SREBF-mediated lipogenesis. (C) Gene set enrichment analysis indicated FGF21 LKO even reduced insulin signaling in OVX mice compared to the sham controls. *** p < 0.001.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Concentration Assay

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 7. The potential mechanism and key genes by which FGF21 LKO abrogated OVX-induced central obesity in mice. (A) The potential mechanism by which FGF21 LKO abrogated OVX-induced central obesity in mice. Liver-specific FGF21 knockout reduced both GC and insulin production, which in turn decreased adipogenesis and lipogenesis in visceral adipose tissues. (B) The potential key genes mediating FGF21 LKO on abrogating OVX-induced central obesity in mice.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out

Journal: Advanced Science

Article Title: A Time‐Programmed Bilayer Wound Dressing for Dynamic Microenvironment Modulation and Full‐Thickness Regeneration in Diabetic Wounds

doi: 10.1002/advs.202512425

Figure Lengend Snippet: Polyphenols scavenge AGEs and inflammatory factors. Gene set enrichment analysis (GSEA) of (A) defense response, (B) negative regulation of IFN‐I mediated signaling pathway, (C) negative regulation of IL‐6, and (D) activation of innate immune response. Differences in (E) AGEs, (F) RAGE, (G) IL‐4, (H) IL‐6, (I) IL‐10, and (L) TNF‐α levels between groups Control, T2DM, and Polyphenol were detected by Elisa. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 6 independent samples for each group, error bars represent mean ± SD.

Article Snippet: Mouse TNF alpha PicoKine ELISA Kit (catalog no. EK0527), Mouse IL‐10 PicoKine ELISA Kit (catalog no. EK0417), Mouse IL‐6 PicoKine ELISA Kit (catalog no. EK0411), Mouse IL‐4 PicoKine ELISA Kit (catalog no. EK0405) were purchased from BOSTER (Wuhan, China).

Techniques: Activation Assay, Control, Enzyme-linked Immunosorbent Assay

Journal: Advanced Science

Article Title: A Time‐Programmed Bilayer Wound Dressing for Dynamic Microenvironment Modulation and Full‐Thickness Regeneration in Diabetic Wounds

doi: 10.1002/advs.202512425

Figure Lengend Snippet: Expression of inflammatory and anti‐inflammatory factors during wound healing. (A) Representative images of immunohistochemical staining of IL‐4, (B) IL‐10, (C) TNF‐α, (D) IL‐6 in Control, Nanofiber, Polyhenol, PDGF‐BB, and Polyhenol+PDGF‐BB groups at 3 and 7 days. (E–H) Quantification of IL‐4, IL‐10, TNF‐α, and IL‐6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Mouse TNF alpha PicoKine ELISA Kit (catalog no. EK0527), Mouse IL‐10 PicoKine ELISA Kit (catalog no. EK0417), Mouse IL‐6 PicoKine ELISA Kit (catalog no. EK0411), Mouse IL‐4 PicoKine ELISA Kit (catalog no. EK0405) were purchased from BOSTER (Wuhan, China).

Techniques: Expressing, Immunohistochemical staining, Staining, Control

Journal: International Journal of General Medicine

Article Title: Characterization of Gastric Mucinous Carcinoma: Clinicopathological Insights and Vascular Features via MSCT

doi: 10.2147/IJGM.S567353

Figure Lengend Snippet: Immunohistochemistry (IHC) detection of vascular endothelial growth factor A (VEGF-A), hypoxia-inducible factor-1α (HIF-1α), and epidermal growth factor (EGF) in carcinomas and corresponding paracancerous normal tissues of MGC, LEMPC, and NMGC. ( A – C ) Representative immunohistochemical staining of VEGF-A ( A ), HIF-1α ( B ), EGF( C ) in carcinomas and normal tissues (×400, bar = 50μm). ( D – F ) IHC score differences (cancer tissue score - normal tissue score) for VEGF-A ( D ) HIF-1α ( E ) EGF ( F ) among the three groups. Statistical significance is indicated as follows: P < 0.05; P < 0.001.

Article Snippet: Sections were stained for CD34 (1:2500, Abcam, ab81289), VEGF-A (1:800, Proteintech, 66828-1-IG), HIF-1α (1:200, Proteintech, 20960-1-AP), and EGF (1:200, Proteintech, 27141-1-AP).

Techniques: Immunohistochemistry, Immunohistochemical staining, Staining

Journal: GeroScience

Article Title: Low protein-induced-FGF-21 signaling remodels adipose tissue on reduced markers of senescence during aging

doi: 10.1007/s11357-025-01853-w

Figure Lengend Snippet: Low-protein diet late in life prevents age-related and obesity-related metabolic decline. A Graphical methodology: C57BL/6 (WT) male mice were placed on CON, LP, HFCON, and HFLP at 16 months of age (8–12 mice/group), various metabolic endpoints on BW and glucose homeostasis throughout the feeding phase of the study as indicated, and tissue collection at 22 m of age. B Body weight gain over time from initiation of diet. C Terminal body weight gain at 22 months of age. D Fat gain at 22 months of age. E Lean gain at 22 months of age. F Fasting blood glucose at 21 months of age. G Glucose tolerance test conducted at 21 months of age ( n = 9 mice/diet). H Area under the curve glucose for the GTT. I Serum FGF21 levels at 22 months of age. J Serum adiponectin levels at 22 months of age. Statistical analyses were conducted using one-way ANOVA. All values are mean ± SEM, with significant main effects of protein or post hoc comparison within the fat*protein interaction

Article Snippet: Serum concentrations of FGF21 (no. RD291108200R, Mouse and Rat FGF-21 ELISA, BioVendor) and adiponectin (#EZMADP-60 K, Mouse Adiponectin EMD Millipore Corporation) were determined via ELISA according to the manufacturer’s recommended protocol.

Techniques: Comparison

Journal: GeroScience

Article Title: Low protein-induced-FGF-21 signaling remodels adipose tissue on reduced markers of senescence during aging

doi: 10.1007/s11357-025-01853-w

Figure Lengend Snippet: Low-protein diet induces FGF21-dependent transcriptional signatures on improved thermogenesis and reduced markers on cell senescence in white adipose tissue. Inguinal WAT and epididymal WAT, from a previous study, were used to perform bulk RNA-seq from C57BL/6 (WT) and Fgf21 KO mice fed either normal-protein control (CON) and low-protein (LP) diet at 3 months of age until 22 months of age. A Graphical methodology: subcutaneous (iWAT), visceral (gWAT), and brown (BAT) adipose tissue from a previous study of C57BL/6 (WT) and Fgf 21 KO mice fed either normal-protein (CON) or low-protein (LP) at 3 months of age until 22 months of age. B–D Inguinal WAT. B PGSEA: Parametric Gene Set Enrichment Analysis for GO: Biological Processes. C Heatmap of top DEGs in CON and LP fed WT mice. D Heatmap of top DEGs in LP-fed WT mice and Fgf21 KO mice. E–G Epididymal WAT. E PGSEA: Parametric Gene Set Enrichment Analysis for GO: Biological Processes. F Heatmap of top DEGs in CON and LP fed WT mice. G Heatmap of top DEGs in LP-fed WT mice and Fgf21 KO mice. Heatmaps are listed by FDR < 0.05, and color key shows logFC 2 heatmaps. Teal blue represents downregulated, and lavender represents upregulated enriched genes within the comparison groups

Article Snippet: Serum concentrations of FGF21 (no. RD291108200R, Mouse and Rat FGF-21 ELISA, BioVendor) and adiponectin (#EZMADP-60 K, Mouse Adiponectin EMD Millipore Corporation) were determined via ELISA according to the manufacturer’s recommended protocol.

Techniques: RNA Sequencing, Control, Comparison

Journal: GeroScience

Article Title: Low protein-induced-FGF-21 signaling remodels adipose tissue on reduced markers of senescence during aging

doi: 10.1007/s11357-025-01853-w

Figure Lengend Snippet: Low-protein diet induces fgf21-dependent transcriptional signatures on improved vascular-related remodeling in brown adipose tissue. BAT from a previous study was used to perform bulk RNA-seq from C57BL/6 (WT) and Fgf21 KO mice fed either normal-protein control (CON) and low-protein (LP) diet at 3 months of age until 22 months of age. A PGSEA: Parametric Gene Set Enrichment Analysis for GO: Biological Processes. B Heatmap of top DEGs in CON and LP fed WT mice. C Heatmap of top DEGs in LP fed WT mice and Fgf21 KO mice. Heatmaps are listed by FDR < 0.05, and color key shows logFC 2 heatmaps. Teal blue represents downregulated, and lavender represents upregulated enriched genes within the comparison groups

Article Snippet: Serum concentrations of FGF21 (no. RD291108200R, Mouse and Rat FGF-21 ELISA, BioVendor) and adiponectin (#EZMADP-60 K, Mouse Adiponectin EMD Millipore Corporation) were determined via ELISA according to the manufacturer’s recommended protocol.

Techniques: RNA Sequencing, Control, Comparison